Studying how to enable humans to survive in an oxygen-free environment through gene regulation is a very challenging and forward-looking topic. Here are some possible research directions and aspects to consider: 1. **Understanding the mechanism of hypoxia adaptation**: Study certain organisms that can survive in low-oxygen or oxygen-free environments, such as certain bacteria, yeast, or deep-sea organisms. Genome analysis of these organisms can provide clues about the mechanism of anaerobic survival. 2. **Enhancing hypoxia tolerance through genetic engineering**: Identify and modify human genes related to hypoxia tolerance. For example, study the HIF (hypoxia inducible factor) signaling pathway related to hypoxia response, or the potential adaptation of retrotransposon genes to hypoxia. 3. **Metabolic regulation**: Optimize the metabolic pathways of cells to reduce the demand for oxygen. For example, increase the efficiency of the intracellular glycolysis pathway so that it can still effectively produce energy in the absence of oxygen. 4. **Application of synthetic biology**: Use synthetic biology to design and synthesize new metabolic pathways or synthesize new physiologically active substances so that human cells can maintain activity without oxygen. 5. **Simulation technology and experimental verification**: Create methods to simulate low-oxygen or anaerobic environments under laboratory conditions to test genetic engineering schemes and their effects on human cells. This requires rigorous ethical review and safety considerations. 6. **Ethical and safety considerations**: The application of gene editing technology in humans must undergo strict ethical approval, and consider the risks associated with gene editing and possible social ethical issues in advance. 7. **Multidisciplinary collaboration**: Collaborate with experts in biology, medicine, engineering, astronomy and other fields to achieve interdisciplinary breakthroughs from theoretical research to practical applications. Exploring the impact of survival in an anaerobic environment on the human body and actively studying possible gene regulation schemes will provide important support for future deep space travel and human exploration. However, due to its complexity and potential risks, research in this area needs to be conducted with caution.