The use of animals in experiments to test the effect of extreme pressure requires strict ethical and scientific norms to ensure the scientific nature of the experiment and the welfare of animals. The following is a possible research framework： ### 1. **Clarify the purpose of the research** -Determine specific goals for research, such as testing the physiological, behavioral, or psychological effects of extreme stress on animals. -Determine the indicators that need to be measured, such as heart rate, blood pressure, hormone levels, behavioral changes, etc. ### 2. **Choose the appropriate animal model** -Select a suitable animal model according to the research purpose. For example, mice, rats, monkeys, etc. are commonly used in stress-related research. - Ensure that the selected animal model can simulate the human response under extreme pressure. ### 3. **Design experimental program** -**Pressure source design**: Select appropriate pressure sources, such as electric shock, noise, social isolation, forced swimming, etc., to simulate the extreme pressure environment. -**Experimental group and control group**: Set up an experimental group and a control group. The experimental group accepts stressors, and the control group does not accept stressors or accepts slight stressors. -**Timing**: Determine the duration of stress exposure, such as short-term (acute stress) or long-term (chronic stress). ### 4. **Implement experiments** - **Animal adaptability training**: Before the experiment begins, let the animals adapt to the experimental environment to reduce the stress response. -**Stress exposure**: According to the experimental design, a stress source is applied to the animals in the experimental group. -**Data collection**: During and after the experiment, collect relevant data, such as physiological indicators, behavioral observations, blood samples, etc. ### 5. **Data analysis** - **Physiological data analysis**: Analyze the effects of stress on animal physiological indicators, such as heart rate, blood pressure, hormone levels, etc. - **Behavioral data analysis**: Observe and record changes in animal behavior, such as anxiety, depression, aggressiveness, etc. -**Comparative analysis**: Compare the data of the experimental group and the control group to evaluate the effect of the ultimate pressure. ### 6. **Ethical review and Animal welfare** - **Ethical review**: Ensure that the experimental program has passed the review of the Animal Ethics Committee and complies with relevant laws and regulations. -**Animal welfare**: During the experiment, minimize the pain and discomfort of the animals, provide appropriate care and a comfortable environment. ### 7. **Explanation and report of results** -**Explanation of the results**: According to the results of the data analysis, explain the effects of extreme pressure on animals. -**Research report**: Write experimental reports, record experimental design, methods, results and conclusions in detail for peer review and academic exchanges. ### 8. **Follow-up research** -**Verification experiment**: If necessary, repeat the experiment to verify the reliability of the results. -**Further research**: Based on the experimental results, design further research to explore the mechanism of extreme stress or find interventions. ### Precautions： -**Principles of substitution, reduction, and optimization**: In animal experiments, the principle of “3R”, namely Replacement, Reduction, and Refinement, should be followed to minimize the number and pain of animals used. -**Compliance**: Ensure that the experiment complies with the laws, regulations and ethics of the country or region where it is located. Through the above steps, animal models can be used to study the effects of extreme stress within the framework of science and ethics, and valuable scientific data can be provided for human health and related fields.